Nearly £500 was stolen from the St Andrews Botanic Garden after it was robbed twice in one week.
The garden on Cannongate, which was saved from closure only last year, is owned by the University and run by a charitable trust that took over from Fife Council last year.
Garden education officer Nikki Macdonald told The Courier that the incidents shocked staff and volunteers.
In addition to the lost cash, some of which was a collection for the Learning Den project, she said repairs and lock changes were costly matters.
She said: “We are operating on a shoestring and things like this can tip the balance. We have had to tighten up security.”
The police are currently investigating the matter.
A police spokeswoman told The Saint: “Police in Fife were called to St Andrews Botanic Gardens on Monday 6 April following reports of an attempted housebreaking.”
“Anyone with information regarding this incident is urged to contact Police Scotland on 101.